相关实验视频
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In vitro Reconstitution of the Active T. castaneum Telomerase
Published on: July 14, 2011
对于端粒酶RNA的一种保存的二次结构.
1Department of Microbiology and Immunology, University of California, San Francisco 94143.
Cell
|October 18, 1991
概括
泰隆玛酶RNA是什么意思
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 端粒酶是维持端粒长度所必需的一种核糖蛋白酶.
- 端粒酶的RNA成分作为端粒DNA合成的模板.
- 了解端粒酶RNA结构对于理解其功能至关重要.
研究的目的:
- 确定多种状动物中端粒酶RNA (TER) 的二次结构.
- 在TER中识别对端粒DNA合成至关重要的保存结构元素.
- 构建TER二次结构的比较系遗传模型.
主要方法:
- 来自七种四胺纤维动物的TER基因的家族遗传学比较分析.
- 克隆和测序来自Tetrahymena malaccensis,T. pyriformis,T. hyperangularis,T. pigmentosa,T. hegewishii和Glaucoma chattoni的TER基因. 这种基因的复制和测序.
- 通过假定基数对的共变分析识别同源补充序列.
主要成果:
- 在所有分析的端粒酶RNA中发现了一个高度保守的二次结构,尽管主要序列的快速分歧.
- 发现了一个由22个完全保存的核酸组成的块,包含了端粒模板区域.
- 共变分析揭示了同源的互补序列,支持了拟议的二次结构模型.
结论:
- 端粒酶RNA的二次结构在各种状动物物种中得到了显著的保存.
- 保存的结构,特别是模板区域,对于端粒DNA合成中的端粒酶功能至关重要.
- 这项研究为端粒酶RNA提供了一个强大的二次结构模型.
相关概念视频
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
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Telomeres and Telomerase
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Telomeres and Telomerase
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
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DNA Structure
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