一种由巨类细胞分泌的肝素结合生长因子,与EGF相关
S Higashiyama1, J A Abraham, J Miller
1Department of Surgical Research, Children's Hospital, Boston, MA 02115.
概括
研究人员发现了一种由巨细胞分泌的新型肝素结合生长因子 (HB-EGF). 这种强大的辅酶原体刺激了光滑肌肉细胞的增殖,这表明它在巨驱动的细胞生长中发挥了作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 巨类U-937细胞分泌各种生长因子.
- 识别新型线粒原体对于理解细胞增殖至关重要.
- 皮表皮生长因子 (EGF) 家族在细胞生长和分化中起着重要作用.
研究的目的:
- 为了描述U-937细胞分泌的新发现的22千多肝素结合生长因子.
- 为了确定这种新型因子的代潜力和受体结合.
- 为了研究这种因素在巨细胞介导的细胞增殖中的作用.
主要方法:
- 补充DNA (cDNA) 克隆和序列分析以预测氨基酸序列.
- 使用BALB-3T3纤维细胞,光滑肌细胞和内皮细胞进行线粒性测定.
- 使用A-431表皮癌细胞和光滑肌肉细胞进行约束性研究,以评估受体相互作用.
主要成果:
- 确定了一种22千多的肝素结合生长因子HB-EGF.
- HB-EGF是EGF家族的一种新成员,对纤维细胞和光滑肌细胞具有线粒性作用,但不是内皮细胞.
- HB-EGF与EGF受体结合,是比EGF更强大的平滑肌肉细胞的线粒原体.
- 在人体巨细胞中表达HB-EGF.
结论:
- HB-EGF是一种新型的肝素结合生长因子,对光滑肌肉细胞具有显著的线粒体效应.
- 与EGF相比,HB-EGF对光滑肌肉细胞的强烈菌效应凸显了其独特的生物作用.
- 在巨细胞中HB-EGF的表达表明它参与了巨细胞介导的细胞增殖,并可能参与炎症或伤口愈合过程.
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