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基本的自我裂变型III分泌蛋白Escu和SpaS的结构分析

Raz Zarivach¹, Wanyin Deng, Marija Vuckovic

¹Department of Biochemistry and Molecular Biology, and the Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada.

|May 3, 2008

概括

格拉姆阴性细菌中的III型分泌系统 (T3SS) 使用一个分子开关,Escu,它经历了自动分裂. 这种裂变调节了毒性因子向宿主细胞的分泌.

科学领域:

微生物学微生物学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

第三类分泌系统 (T3SS) 对于格拉姆阴性细菌的致病性至关重要,它可以直接注射效应蛋白到宿主细胞中.
T3SS组装涉及复杂的蛋白质结构,跨越细菌膜和宿主细胞膜,由蛋白质管连接在一起.
在T3SS和鞭毛组装中,一个关键的调节步骤是内膜蛋白的翻译后自动分裂,如Escu/FlhB.

研究的目的:

研究Escherichia coli中Escu自动裂变的机制和结构影响.
阐明ESCU自动断在调节T3SS组装和功能的作用.
为了获得对阿斯巴拉金循环介导的自我切割反应的结构洞察力.

主要方法:

对大肠杆菌Escherichia coli EscU蛋白的结构分析.
在体内研究使用Escu的点和删除突变.
生物化学分析以描述自动裂变机制.

主要成果:

确定了Escu表面的II型β转向作为自动裂变的位置.
证明自动裂变涉及一个经过循环的保存的氨酸残留物.
展示了自动裂变如何微妙地改变 EscU 形状,影响与其他 T3SS 组件的相互作用.

结论:

EscU自动裂变作为一个分子开关,调节T3SS组装和效应器分泌.
这项研究揭示了阿斯巴拉金在蛋白质自我分裂中的新型循环机制.
调查结果为T3SS监管的结构动态提供了结构性的见解.

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