富含U的管道增强了酵母酵母中替代3'拼接位置的使用
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|January 11, 1991
概括
酵母菌的拼接机制与哺乳动物的机制没有根本的区别. 富含尿素的管道增强了3'拼接位的竞争,影响了替代拼接位的选择.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA分离过程中.
背景情况:
- 哺乳动物和酵母的拼接机制被认为在3'拼接部位识别方面有显著差异.
- 在哺乳动物拼接中,前面3'拼接部位的富含胺的图案至关重要.
研究的目的:
- 调查尿素含量在酵母替代3'拼接部位选择中的作用.
- 为了确定酵母拼接是否需要类似于哺乳动物的金字胺丰富的图案.
主要方法:
- 使用体内测试来测试尿素含量对酵母合点竞争的影响.
- 分析了具有不同尿素含量和近距离的替代3'拼接部位.
主要成果:
- 在PyAG序列之前的富含尿素的管道显著增强了其作为酵母中的拼接受体的功能.
- 如果它具有优越的序列上下文,可以比近距离的PyAG更受青,从而挑战简单的扫描模型.
- 缺少上游尿素的远端3'拼接部位是第二个拼接步骤的低效基质.
结论:
- 酵母拼接,就像哺乳动物拼接一样,受益于富含尿素的序列,以有效地识别3'拼接部位.
- 富含尿素的图案对于在酵母菌中正确识别和利用远端拼接部位至关重要.
- 这些发现表明,跨物种的3'拼接位点选择有保存的机制.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


