相关实验视频
Updated: May 11, 2026
08:30
RNAi Interference by dsRNA Injection into Drosophila Embryos
Published on: April 11, 2011
一个内源的小干扰RNA途径在Drosophila
Benjamin Czech1, Colin D Malone, Rui Zhou
1Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.
Nature
|May 9, 2008
概括
德罗斯菲拉具有第三个小RNA类,内源性小干扰RNA (esiRNAs),在淋巴体和体质组织中发现. 这些esiRNAs与microRNAs和Piwi相互作用的RNAs不同,它们结合Argonaute-2并调节基因表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 类植物利用微RNA (miRNA) 和Piwi相互作用RNA (piRNA) 等小RNA进行基因调控和转子子沉默.
- miRNAs由Drosha-Pasha和Dicer-1-Loquacious进行处理,结合阿尔戈诺-1.
- piRNAs是性腺特异的,结合Piwi蛋白质,并通过Piwi催化裂变生成.
研究的目的:
- 鉴定和描述Drosophila的一种新型小RNA类型.
- 为了阐明这个新的小RNA类的生物发生和阿尔戈诺特蛋白质关联.
- 确定Drosophila中的第三个小RNA类的功能影响.
主要方法:
- 在Drosophila组织中分析小RNA种群.
- 涉及Dicer-2和Loquacious的基因操纵,以研究esiRNA生物发生.
- 鉴定新型小RNAs的阿尔戈诺特蛋白合作伙伴.
- 基因组位置和转录模式的表征产生这些RNA.
主要成果:
- 发现了第三种内源性小RNA类:内源性小干扰RNA (esiRNAs).
- esiRNA生产需要Dicer-2,一些子集更喜欢Loquacious而不是R2D2.
- esiRNAs是由融合转录单元和结构化基因组基因位点以特定组织的方式生成的.
- 这些esiRNAs主要与Argonaute-2相关,并且可以针对蛋白质编码基因和移动元素.
结论:
- 果虫至少有三个不同的内源性小RNA类.
- 新发现的esiRNAs代表了一个桥梁已知的生物发生路径和功能角色的类.
- 这一发现扩大了对Drosophila中小RNA多样性和调节机制的理解.
相关概念视频
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
02:57piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
02:30Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...