通过结合的CDK和网络振荡器对细胞循环转录进行全球控制
David A Orlando1, Charles Y Lin, Allister Bernard
1Department of Biology, Duke University, Durham, North Carolina 27708, USA.
Nature
|May 9, 2008
概括
发芽的酵母基因表达是为细胞分裂定时的. 循环素依赖性激酶 (CDK) 对此有影响,但转录因子网络也独立地驱动周期性基因转录.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 基因表达时间对于Saccharomyces cerevisiae细胞周期进展至关重要.
- 转录因子网络和循环素依赖激酶 (CDK) 参与调节细胞循环依赖转录.
- 以前的研究表明,CDK活动主要控制周期性基因转录.
研究的目的:
- 研究环林-CDK复合体在控制全球细胞循环转录程序中的作用.
- 确定CDK是否完全负责在细胞周期期间建立周期性转录程序.
主要方法:
- 基因组范围的转录动力学分析了缺乏S相和线性环林的初芽酵母突变体.
- 在这些缺乏环林的突变体中,监测了细胞周期进展.
主要成果:
- 很大一部分定期表达的基因在环林突变中表现出异常表达.
- 尽管细胞周期在G1/S边界停止,但大约70%的周期性基因保持了预定的周期性表达.
- 环林-CDK活性不是全球周期转录计划的唯一决定因素.
结论:
- 循环素依赖性激酶在细胞循环转录调节中发挥作用,但并不完全控制整个程序.
- 周期性基因转录似乎是转录因子网络的新兴属性.
- 这种转录因子网络可以作为细胞周期振荡器,独立或与CDK振荡器协同工作.
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