IL-21和TGF-β是人类T(H) 17细胞分化所需的
Li Yang1, David E Anderson, Clare Baecher-Allan
1Division of Molecular Immunology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature
|May 13, 2008
概括
转化生长因子-β (TGF-β) 和IL-21独特地将原始人类CD4 ((+)) T细胞分化为T ((H)) 17细胞. 这些发现有助于我们更好地了解人类自身免疫性疾病中的T(H) 17细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 干白素-17 (IL-17) 分泌T辅助细胞17 (T) 和调节性T细胞 (T) 在免疫反应中至关重要.
- T(H) 17细胞与多发性硬化症和类风湿性关节炎等自身免疫性疾病有关.
- 小鼠T(H) 17细胞分化是由TGF-β和IL-6或TGF-β和IL-21诱导的,而IL-23则稳定了表型.
研究的目的:
- 阐明驱动人类天真CD4的T细胞分化成TH17细胞的因素.
- 为了区分人类T(H) 17细胞生成与小鼠模型.
- 为了确定人类T(H) 17细胞发育的关键细胞因子.
主要方法:
- 研究了各种细胞因子 (IL-1β,IL-6,TGF-β,IL-21) 在人体CD4+) T细胞分化中的作用.
- 利用了来自人体外围血液的天真和中央记忆CD4 (((+) T细胞种群.
- 评估IL-17A分泌和RORC2转录因子表达.
主要成果:
- IL-1β和IL-6诱导了人体中央记忆CD4 (((+) T细胞中的IL-17A分泌.
- TGF-β和IL-21独特地促进了人类原始CD4 ((+) T细胞的分化为T ((H)) 17细胞.
- 这种分化伴随着转录因子RORC2.2的表达.
结论:
- TGF-β和IL-21是分化人类原始CD4 ((+) T细胞成T ((H)) 17细胞的关键因素.
- 这些发现突出了人类和小鼠T(H) 17细胞分化途径之间的差异.
- 鉴定到的T(H) 17细胞群为研究人类炎症疾病提供了新的途径.
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