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皮质-介质细胞过渡产生具有干细胞特性的细胞
Sendurai A Mani1, Wenjun Guo, Mai-Jing Liao
1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA. smani@mdanderson.org
Cell
|May 20, 2008
概括
皮质-介质细胞过渡 (EMT) 与乳腺癌中的干细胞特性有关. 在乳腺细胞中诱导EMT可增强干细胞标记物和乳球形成,这对癌症进展至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学 发展生物学
- 癌症研究 癌症研究
背景情况:
- 表皮-介质细胞过渡 (EMT) 是一个关键的发育过程,与癌症入侵和转移有关.
- 了解EMT的分子基础及其与干细胞特性之间的关系对于癌症治疗至关重要.
研究的目的:
- 调查EMT诱导与人类乳腺上皮细胞中干细胞特性获得之间的联系.
- 为了确定干细胞类细胞是否表现出EMT标记物,以及EMT转化细胞是否具有增强的瘤形成能力.
主要方法:
- 在不朽化的人类乳腺上皮细胞 (HMLEs) 中诱导EMT.
- 对介质细胞特征和干细胞标记物表达的分析.
- 乳腺球形成测试以评估干细胞类属性.
- 干细胞样种群的隔离和表征.
- 在EMT转化细胞中评估瘤发生潜力.
主要成果:
- 在HMLEs中诱导EMT导致介质细胞特征和干细胞标记物表达.
- 接受EMT的细胞显示乳球形成增加,这是乳腺干细胞的标志.
- 孤立的干细胞样细胞表达了EMT标记物,形成了乳球.
- 具有EMT特征的转化乳腺上皮细胞表现出增强的乳球形成,软菌殖民地形成和瘤性.
结论:
- 在上皮-介质细胞过渡 (EMT) 和获得上皮干细胞特性之间存在直接联系.
- EMT可能有助于癌症干细胞的产生和进展.
- 这些发现提供了有关癌症发展和潜在治疗点的见解.
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