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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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相关实验视频

Updated: Jul 5, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

用于细胞成像应用的两种颜色的RNA间探针.

Nathan Stevens1, Naphtali O'Connor, Harshad Vishwasrao

  • 1Departments of Chemistry and Chemical Engineering, Columbia University, New York, New York 10027, USA.

Journal of the American Chemical Society
|May 21, 2008
PubMed
概括

这项研究引入了一种具有增强光的新型RNA探针. 探测器更亮,在蜂环境中提供了改进的信号检测,从而实现双色成像.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 光光谱学 光光谱学

背景情况:

  • 芬丁衍生物被用作核酸的光探针.
  • 提高光信号强度和改善信号与背景比率对于敏感检测至关重要.
  • 开发双色探头可以同时提供探头定位和目标分子存在的信息.

研究的目的:

  • 开发一种更明亮的基于氨酸的光探针,用于双重RNA检测.
  • 通过与光素的共价连接来增强探针的光信号强度.
  • 利用时间分辨率光来改善细胞成像中的信号与背景比率.

主要方法:

  • 合成一种氨酸衍生物与一种共价连接的光素分子.
  • 稳态和时间分辨率的光谱学来描述探头的特性.
  • 细胞的光成像,以评估探针在生物环境中的性能.

主要成果:

  • 这种新型探测器的能量转移效率大约是77%的,从光素转移到光素.
  • 探测器在与RNA结合时比其他类型的氨酸衍生物亮5倍以上.
  • 时间分辨的光使细胞生长介质的信号与背景比率从7增加到40以上.
  • 双色光成像显示了探针位置和RNA的同时可视化.

更多相关视频

Precise Visualization of Insulin Receptors A and B in Murine Brain with an RNA In Situ Hybridization Assay
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Precise Visualization of Insulin Receptors A and B in Murine Brain with an RNA In Situ Hybridization Assay

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相关实验视频

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Precise Visualization of Insulin Receptors A and B in Murine Brain with an RNA In Situ Hybridization Assay
08:34

Precise Visualization of Insulin Receptors A and B in Murine Brain with an RNA In Situ Hybridization Assay

Published on: July 15, 2025

结论:

  • 开发的光素-南丁合物是双重RNA的高度敏感和明亮的探针.
  • 时间分辨的光显著提高了复杂的生物样本中的信号检测.
  • 探测器的双色功能允许同时跟踪探测器定位和RNA存在,提供先进的细胞成像应用.