表面增强共振拉曼光谱对蛋白质原生结构的表征
Manliang Feng1, Hiroyasu Tachikawa
1Department of Chemistry, Jackson State University, 1400 Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217, USA.
Journal of the American Chemical Society
|May 21, 2008
概括
表面增强共振拉曼散射 (SERRS) 揭示了由于蛋白质-纳米粒子相互作用而导致的met-myoglobin (metMb) 的结构变化. 一个新的拉曼流系统保存了metMbMb.
科学领域:
- 生物物理化学 生物物理化学
- 频谱学是一种光谱学.
- 纳米技术纳米技术
背景情况:
- 生物分子的表面增强共振拉曼散射 (SERRS) 光谱往往不同于它们的本源共振拉曼 (RR) 光谱.
- 这些差异归因于纳米粒子相互作用和实验条件引起的分子结构变化.
- 甲基球蛋白 (metMb) 作为一种模型蛋白质来研究这些光谱差异.
研究的目的:
- 确定和量化导致SERRS和RR光谱之间差异的因素.
- 开发一种获得精确反映原生蛋白质结构的SERRS光谱的方法.
- 评估自家设计的拉曼流系统对蛋白质SERRS分析的实用性.
主要方法:
- 使用自家设计的拉曼流系统进行SERRS测量甲基肌球蛋白.
- 研究了蛋白质-纳米粒子相互作用持续时间和激光照射对光谱特征的影响.
- 用流量系统获得的SERRS光谱与传统的SERRS和RR光谱进行了比较.
- 分析了与metMb的血红素口袋结构和协调状态有关的光谱变化.
主要成果:
- 蛋白质纳米粒子相互作用和激光照射都会导致metMb的结构变化,导致SERRS/RR光谱差异.
- 在纳米颗粒上长时间的蛋白质吸附会破坏血口袋,导致远端水分子的损失,并形成一个5协调的高旋转血.
- 拉曼流系统,在最佳流速下,最大限度地减少结构干扰,同时保持高SERRS增强.
- 使用流量系统获得的metMb的SERRS光谱与原生RR光谱密切匹配,即使度明显较低.
结论:
- 蛋白质-纳米粒子相互作用的程度和激光照射是影响蛋白质SERRS光谱的关键因素.
- 拉曼流系统通过减轻有害相互作用,在SERRS分析期间有效地保留本地蛋白质结构.
- 这种流系统对于精确的SERRS蛋白质-纳米粒子相互作用和蛋白质的原生结构的表征是有价值的.
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