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Updated: May 10, 2026
11:03
Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
Published on: December 22, 2014
这种trk原型瘤基因编码了神经生长因子的受体
1Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
Cell
|April 5, 1991
概括
trk原型瘤基因产物gp140trk作为高亲和度神经生长因子 (NGF) 受体起作用. 该受体介导NGF信号传递,包括铁酸化和激酶活性,独立于非神经细胞中的低亲和受体.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 存在两种不同的神经生长因子 (NGF) 受体:一种是低亲和度受体 (LNGFR),另一种是高亲和度受体,其结构尚不清楚.
- 低亲和度受体是一个由LNGFR基因编码的氨酸丰富的糖蛋白.
研究的目的:
- 为了确定高亲和度NGF受体的结构性质.
- 研究trk原瘤基因产品 (gp140trk) 在NGF结合和信号传递中的功能作用.
主要方法:
- 化学交叉链接实验检测NGF与gp140trk结合.
- 在各种细胞类型 (PC12,小鼠纤维细胞,昆虫Sf9细胞) 中分析gp140trk的表达.
- 在NGF刺激后评估gp140trk氨酸酸化和氨酸激酶活性.
主要成果:
- 人类trk原型瘤基因产物gp140trk以高亲和力 (Kd~10^-11到10^-10 M) 结合NGF.
- 在多种细胞类型中,NGF可以与gp140trk交叉链接,包括那些缺乏LNGFR的细胞类型.
- NGF刺激诱导了快速的氨酸酸化,并激活了gp140trk的氨酸激酶活性.
结论:
- gp140trk是一种功能高亲和度NGF受体.
- gp140trk调解了关键的NGF信号转导通路,包括氨酸激酶激活.
- 高亲和度NGF与gp140trk结合可以在非神经细胞中独立于LNGFR发生.
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