髓素生物合成:直接证据表明,载体蛋白结合的乙烯减少了2和4个电子
Yanyan Li1, Kira J Weissman, Rolf Müller
1Pharmaceutical Biotechnology, Saarland University, P.O. Box 151150, 66041 Saarbrücken, Germany.
Journal of the American Chemical Society
|May 24, 2008
概括
研究人员在体外成功合成了myxochelin B,揭示了 siderophore 生物合成中的一个关键的化物中间体. 这一发现澄清了菌中的铁获取途径,并确定了控制素A和B比率的酶.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 微生物利用 siderophores 获得铁.
- 甲素A和B是由Stigmatella aurantiaca*产生的甲酸化体.
- 以前的研究测序了myxochelin基因集群,但未能在体外复制myxochelin B生物合成.
研究的目的:
- 为了在体外实现肌素B的生物合成.
- 为了证明在 siderophore 合成中存在一种化物中间体.
- 阐明对肌素A和B生产的酶控制.
主要方法:
- 在实验室中,素生物合成途径的复制.
- 使用纯化的成分进行酶分析.
- 使用生化技术分析反应中间体.
主要成果:
- 在体外成功生物合成了myxochelin B.
- 在降低基载体蛋白结合化物的过程中,证明一种过渡性化物中间体.
- 确定MxcL和MxcG还原酶域之间的竞争,以确定阿尔代中间体.
结论:
- 这项研究提供了第一个体外证据,证明了myxochelin B生物合成.
- 在 siderophore 合成的还原性途径中确认了一种化物中间体.
- 酶竞争调节了肌素A与肌素B的比例.
相关概念视频
Role of Reduced Coenzymes NADH and FADH₂
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
Electron Transport Chains
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
Electron Transport Chain: Complex III and IV
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
Oxidation and Reduction of Organic Molecules
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
The removal of an electron from a molecule, results in a...
Electron Transport Chain: Complex I and II
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
Electron Carriers
Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...


