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相关概念视频

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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相关实验视频

Updated: Jul 4, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

通过整合性基因组分析剖析直接重编程.

Tarjei S Mikkelsen1, Jacob Hanna, Xiaolan Zhang

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Nature
|May 30, 2008
PubMed
概括
此摘要是机器生成的。

将体细胞重新编程为多能性,涉及复杂的基因表达变化. 部分重编程的细胞因不完整的因子抑制和低效的DNA脱甲基化而被困,但RNA抑制和脱甲基化抑制剂提高了效率.

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相关实验视频

Last Updated: Jul 4, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
12:53

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

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科学领域:

  • * 细胞重编程和发育生物学.
  • * 细胞命运转变的基因组和表观遗传学分析.

背景情况:

  • *体细胞可以通过异位转录因子表达恢复多能性.
  • *理解重编程机制是提高效率和安全的关键.

研究的目的:

  • *对小鼠纤维细胞和B淋巴细胞重编程进行综合性基因组分析.
  • * 研究细胞重编程到多能性的机制和动力学.

主要方法:

  • *对小鼠纤维细胞和B淋巴细胞进行重编程的综合基因组分析.
  • *对基因表达,表观遗传状态和转录因子活性进行分析.

主要成果:

  • * 完全重编程的细胞实现了与胚胎干细胞类似的基因表达和表观遗传特征.
  • *部分重编程的细胞在关键位置表现出不完全的转录因子抑制和DNA高甲基化.
  • * 不完整的抑制和低效的DNA脱甲基化阻碍了完全的重编程.

结论:

  • *细胞可以通过不完全的转录因子沉默被困在部分重编程状态中.
  • *DNA脱甲基化是实现多能性的关键,但低效的步骤.
  • *RNA抑制和DNA甲基转移酶抑制剂提高了重新编程的效率.