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相关概念视频

Colloids03:22

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...

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相关实验视频

Updated: Jul 4, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

通过DNA和DNA酶介导的2D合体组件.

Margaret H S Shyr1, Daryl P Wernette, Pierre Wiltzius

  • 1Beckman Institute, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|June 11, 2008
PubMed
概括

DNA杂交使控制的合体自我组装成为有序的二维数组成为可能. 这种DNA介导的组合是可逆的,序列特定的,并且可以被DNAzymes破坏,从而对纳米粒子结构提供精确的控制.

科学领域:

  • 材料科学 材料科学 材料科学
  • 纳米技术 纳米技术
  • 生物化学 生物化学

背景情况:

  • 合体自我组装对于创建先进材料至关重要.
  • 通过DNA介导的相互作用提供了对分子组装的精确控制.
  • 开发特定序列和可逆组装方法是一个关键的挑战.

研究的目的:

  • 为了证明DNA杂交对序列特定的可逆自组合的合式数组.
  • 为了研究DNA介导的体组件的动力学和效率.
  • 探索DNAzymes用于对合体组件的动态控制的使用.

主要方法:

  • 微接触式打印用于空间控制DNA表面图案.
  • 聚乙烯合物与DNA的功能化.
  • 使用激光衍射和光显微镜的监控组件.
  • 热循环以评估可逆性和序列特异性.

主要成果:

  • 通过DNA杂交实现了顺序良好的二维体阵列.
  • 证明了热可逆性 (在50°C化) 和重新组装.
  • 确认了高序列特异性 (<1%的非特异性结合).
  • 获得了组装动力学,显示在6.25小时内完成了90%.

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Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

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Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
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Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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Published on: April 12, 2019

  • 展示了使用DNAzymes在Pb(2+) 存在下破坏组装的过程.

    • 结论:

    • DNA杂交是一种强大的工具,用于控制,序列特定和可逆的合体自我组装.
    • 开发的方法允许对阵列形成进行精确的空间和时间控制.
    • 纳入DNA酶为组装结构的动态调节提供了一种机制.