在RNA中的C2'-endo核酸中缓慢的构造动态
Costin M Gherghe1, Stefanie A Mortimer, Joseph M Krahn
1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.
Journal of the American Chemical Society
|June 19, 2008
概括
在RNA中缓慢的构造变化,使用RNA选择性2′-基化分析,通过初级扩展 (SHAPE) 化学分析,揭示了特定的C2′-endo核酸,作为分子开关. 这些动态对于RNA折叠和功能至关重要.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- RNA分子在广泛的时间尺度上表现出局部结构动态,从皮秒到分钟.
- 较慢的运动对于RNA折叠,配体结合和核糖蛋白组合至关重要,但对大型复杂的RNA研究具有挑战性.
- 通过Primer Extension (SHAPE) 分析的RNA选择性2′-基化,化学探测核酸灵活性,通过监测在利糖2′-基位上的化.
研究的目的:
- 研究局部RNA形态动态的作用,特别是C2'-endo形态,在控制RNA的反应性和功能.
- 用修改的SHAPE化学来描述RNA结构变化的时间尺度.
- 在RNA结构中识别影响生物过程的潜在分子开关.
主要方法:
- 利用RNA SHAPE化学与不同电友性的乙基化试剂来探测RNA结构动力学.
- 在不同的时间域中监测核酸的灵活性和反应性.
- 对模型RNA系统和具有定义的三级结构的大型RNA应用方法.
主要成果:
- 证明C2'-endo核酸对SHAPE试剂的反应不均;反应性取决于试剂的电友性和核酸的构造状态.
- 鉴定出特定的C2'-endo核酸,表现出异常缓慢的构造变化 (约为10^-4s^-1).
- 表明这些缓慢的动态在模型和复杂的生物RNA结构中都可以观察到.
结论:
- 单独的C2'-endo形态并不决定SHAPE的反应性;相反,形态相互转换的动态是关键.
- 某些C2'-endo核酸因其独特的局部动力学而充当慢分子开关.
- 这些缓慢的动态可能在RNA折叠路径和功能机制中发挥着重要的,但被低估的作用.
相关概念视频
01:19RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...