玻拉和激酶奥罗拉a合作激活激酶Plk1并控制线粒体进入
Akiko Seki1, Judith A Coppinger, Chang-Young Jang
1Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.
Science (New York, N.Y.)
|June 21, 2008
概括
玻拉和奥罗拉A (Aur-A) 协同控制G2-M细胞周期过渡. 玻拉促进了Polo样酶1 (Plk1) 的Aur-A介导激活,这对线粒细胞进入体细胞至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 细胞周期调节细胞周期调节
背景情况:
- 细胞增殖依赖于精确的细胞周期过渡.
- 线性循环素驱动胚胎细胞中的G2-M过渡,但体细胞中的触发因子尚不清楚.
研究的目的:
- 为了确定线粒细胞进入体细胞的触发因素.
- 阐明控制G2-M细胞周期过渡的机制.
主要方法:
- 研究波拉和奥罗拉A (Aur-A) 在细胞循环控制中的作用.
- 分析了蛋白质相互作用和激酶激活通路.
主要成果:
- 玻拉和奥尔-A协同作用,以控制G2-M过渡.
- 玻拉在G2阶段积聚,促进Polo样酶1 (Plk1) 的Aur-A介导激活.
- 玻拉调节了Plk1对Aur-A酸化和激活的可访问性,导致循环林依赖的激酶1激活和线粒体进入.
结论:
- 玻拉-奥尔-A复合体是线粒细胞进入体细胞的关键调节者.
- 这一发现为一个关键的,但不太了解的细胞周期事件提供了机制.
更多相关视频
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
06:29Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Published on: July 30, 2020
相关概念视频
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:17Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...