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在体外和细胞内通过近距离生物化检测蛋白质-蛋白质相互作用

Marta Fernández-Suárez1, T Scott Chen, Alice Y Ting

  • 1Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Journal of the American Chemical Society
|June 28, 2008
PubMed
概括

这项研究引入了一种新的近距离生物化方法,用于检测体外和细胞内的蛋白质-蛋白质相互作用. 该技术利用工程生物酶 (BirA) 和受体 (AP) 进行敏感和特定的检测,提供低背景和高分辨率.

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Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...