Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
Electron Transport Chain Components01:29

Electron Transport Chain Components

The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
Protein Transport to the Thylakoids01:22

Protein Transport to the Thylakoids

Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...
Energy to Drive Translocation01:37

Energy to Drive Translocation

Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

When TiO<sub>2</sub> meets pharmaceuticals: Photocatalytic degradation and environmental safety unveiled.

Ecotoxicology and environmental safety·2026
Same author

Evaluation of a human 3D multi-spheroid model derived from SH-SY5Y cells for cytotoxicity testing.

Scientific reports·2026
Same author

Signaling mechanism of the transmembrane energy receptor Aer.

bioRxiv : the preprint server for biology·2026
Same author

The bacterial swarming factor SwrD forms hexameric rings reminiscent of DNA-binding proteins.

bioRxiv : the preprint server for biology·2026
Same author

Lysinoalanine crosslinking in the extracellular flagellar hook of Synergistota.

bioRxiv : the preprint server for biology·2026
Same author

Bacterial internalins exploit E-cadherin to promote head and neck tumor metastasis and drug resistance.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Jul 4, 2026

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
08:33

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

Published on: September 21, 2021

托方加速电子流通过蛋白质.

Crystal Shih1, Anna Katrine Museth, Malin Abrahamsson

  • 1Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|June 28, 2008
PubMed
概括

生物电荷传输通过多步电子道增强. 一个托残留物显著加快了Pseudomonas aeruginosa azurin.金属氧化还原中心之间的电子转移.

更多相关视频

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
12:07

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

相关实验视频

Last Updated: Jul 4, 2026

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
08:33

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

Published on: September 21, 2021

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
12:07

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 频谱学是一种光谱学.

背景情况:

  • 生物能量流依赖于高效的电荷运输在长的分子距离.
  • 由氧化还原活性氨基酸介导的多步电子道化可以提高电荷转移速率.

研究的目的:

  • 量化干预托残留物在促进远距离金属氧化还原中心之间的电子转移中的作用.
  • 为了研究突变性 Pseudomonas aeruginosa azurin 的电子转移机制.

主要方法:

  • 短暂的光学光谱学.
  • 红外光谱学是红外光谱学.
  • 动力学建模 动力学建模
  • 在Pseudomonas aeruginosa azurin的部位导向突变发生.

主要成果:

  • 在Cu(I) 和光激发的Re(I) -二胺之间发生了电子转移,发生在纳米秒.
  • 这一速度是两个数量级快于预测为单步道的19安格斯特罗姆距离的速度.
  • 在位置122的托残留物显著促进了电子转移过程.

结论:

  • 干预的氧化还原活性氨基酸,如托,可以大大提高生物电荷运输速率.
  • 多步电子道化是一种可行的机制,可以有效地在蛋白质中进行远程电子传输.
  • 这一发现对理解生物系统中的能量流动有意义.