DSCAM是一种网林受体,与DCC合作,调解对网林-1的转向反应
Alice Ly1, Anatoly Nikolaev, Geetha Suresh
1Department of Molecular, Cellular, and Developmental Biology, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
Cell
|July 1, 2008
概括
在下面,下面.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 在神经系统发育过程中,脊柱 commissural 轴突在腹部中线上导航.
- 网林-1及其受体DCC是已知的指导线索,但怀疑还有其他组件.
- 唐氏综合征细胞粘附分子 (DSCAM) 是这个过程的候选基因.
研究的目的:
- 为了研究DSCAM在脊柱 commissural轴突指导中的作用.
- 为了确定DSCAM是否作为网-1受体起作用.
- 阐明涉及网林-1和DSCAM的信号通路.
主要方法:
- 对DSCAM在脊柱 commissural轴突上的表达分析.
- 对于DSCAM的网林-1结合测定.
- 使用基因操纵的功能性研究 in vivo 和 in vitro.
- 在Xenopus脊髓神经元扩展培养物中测试DSCAM介导的Netrin-1吸引力.
主要成果:
- DSCAM在脊柱 commissural 轴突上表达,并与netrin-1结合.
- 对于轴突来说,DSCAM是必不可少的,它们可以向内腔中线增长并跨越内腔中线.
- 无论是DSCAM还是DCC,都能独立调解神经元对网-1的转向反应.
- 在Xenopus神经元中的外源DSCAM表达赋予Netrin-1吸引力,独立于DCC.
结论:
- DSCAM 作为网-1 的功能性受体起作用.
- 网林-1/DSCAM信号传输在脊椎动物的指导轴突中起着至关重要的作用.
- 在神经系统发育过程中,DSCAM是中线轴突路径的关键媒介.
相关概念视频
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
01:21Cell Polarization by Rho Proteins
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...