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来自骨髓微环境的特定Jagged-1信号对于新血管化的内皮原生细胞发育是必要的
Sang-Mo Kwon1, Masamichi Eguchi, Mika Wada
1Stem Cell Translational Research Laboratory, RIKEN Center For Developmental Biology/Institute of Biomedical Research and Innovation, Kobe, Japan.
Circulation
|July 2, 2008
概括
通过Jagged-1 (Jag-1) 介导的Notch信号对于新血管化的内皮细胞 (EPC) 是至关重要的. 这项研究突出了Jag-1的特点.
科学领域:
- 细胞生物学 细胞生物学
- 血管生物学 血管生物学
- 再生医学是一种再生医学.
背景情况:
- 内皮原生细胞 (EPCs) 在缺血性疾病中对新血管化至关重要.
- 控制EPC功能的精确信号通路仍然不完全理解.
研究的目的:
- 阐明Jagged-1 (Jag-1) 介导的Notch信号在调节内皮前细胞 (EPC) 动力学和功能的作用.
- 为了研究调节Jag-1信号传递以增强新血管化的治疗潜力.
主要方法:
- 在小鼠中禁用Jag-1信号,以评估其对产后血管生成和EPCs的影响.
- 来自Jag-1淘汰赛 (Jag-1-/-) 和野生型小鼠的EPC的比较分析.
- 使用表皮细胞过度表达诺奇配体的功能获取研究.
- 在EPC移植后对治疗性新血管化的评估.
主要成果:
- 对Jag-1-介导的Notch信号的失活抑制了后肢缺血中的血管生成,通过损害EPC的增殖,生存,分化和动员.
- 来自Jag-1-/-小鼠的EPC与野生型EPC相比,对缺血性新血管化的治疗潜力较低.
- 通过Jag-1介导的Notch信号促进了EPC承诺和增强了新血管化,在Jag-1-/-小鼠中新血管化受损,这些小鼠通过Jag-1-刺激的EPC移植得到拯救.
结论:
- 来自骨髓微环境的特定Jag-1衍生的Notch信号对于EPC介导的血管生成至关重要.
- 针对Jag-1-Notch信号提供了一个有希望的策略来调节治疗性新血管化.
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