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通过"光点击化学"在细菌细胞中对基因编码的含蛋白进行选择性功能化

Wenjiao Song1, Yizhong Wang, Jun Qu

  • 1Department of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260, USA.

Journal of the American Chemical Society
|July 3, 2008
PubMed
概括

研究人员开发了一种基于四醇的光点击化学方法,用于E. coli中的体内基功能化. 这种简单,无毒的方法允许选择性蛋白质修饰和活细胞中的光监测.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...