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相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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相关实验视频

Updated: Jul 4, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

解构绿色光蛋白质的方法

Kevin P Kent1, William Childs, Steven G Boxer

  • 1Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, USA.

Journal of the American Chemical Society
|July 4, 2008
PubMed
概括
此摘要是机器生成的。

研究人员从两个部分重新组装了绿色光蛋白 (GFP):一个大片段和一个合成. 这种新型的半合成方法允许研究蛋白质组合和引入非自然氨基酸.

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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

相关实验视频

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Published on: April 27, 2018

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 绿色光蛋白 (GFP) 是分子生物学中的一个重要工具.
  • 目前用于GFP修改的方法可能很复杂.
  • 分裂GFP系统为蛋白质研究提供了一个潜在的替代方案.

研究的目的:

  • 研究半合成重组GFP的可行性.
  • 描述重新组装的GFP的功能性质.
  • 探索这种方法在引入非自然氨基酸方面的实用性.

主要方法:

  • 一个大型GFP片段 (GFP 1-10) 的重组表达.
  • 合成一个短 (合成GFP 11) 完成GFP结构.
  • 谱分析以比较重新组装和整个GFP.

主要成果:

  • 从两个不同的组件成功地重组了功能GFP.
  • 与完整的GFP相比,证明了相同的吸收和激发状态质子转移动态.
  • 展示了拆卸和替换合成的能力,改变了染色体吸收.

结论:

  • 半合成重组GFP是一种可行的策略.
  • 这种方法为研究β-barrel组装提供了一个多功能平台.
  • 能够将非自然氨基酸纳入GFP结构.