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从诱导的多能干干细胞生成功能性小鼠心肌细胞

Christina Mauritz¹, Kristin Schwanke, Michael Reppel

¹Leibniz Research Laboratories for Biotechnology and Artificial Organs, Carl-Neuberg Strasse 1, 30625 Hannover, Germany.

|July 16, 2008

概括

诱导多能干细胞 (iPS) 可以分化为功能性心肌细胞,类似于胚胎干细胞 (ES). 这一突破使得用于心脏修复和组织工程的自身细胞疗法成为可能.

科学领域:

干细胞生物学干细胞生物学
心血管研究的心血管研究.
再生医学是一种再生医学.

背景情况:

诱导多能干细胞 (iPS) 非常类似于胚胎干细胞 (ES).
特定于患者的iPS细胞系为疾病建模和个性化治疗提供了潜力.
鉴别iPS细胞的心脏分化对于治疗应用至关重要.

研究的目的:

为了比较小鼠iPS细胞克隆与成熟的小鼠ES细胞系的心脏分化潜力.
评估iPS细胞衍生的心肌细胞的分子,结构和功能特征.

主要方法:

基于胚胎体的标准分化协议.
逆转录聚合酶连锁反应 (RT-PCR) 用于基因表达分析.
蛋白质表达的免疫细胞学.
细胞内Ca(2+) 成像和电生理学研究.

主要成果:

iPS细胞分化为心肌细胞,55%的胚胎体呈现自发收缩.
从iPS细胞衍生的心肌细胞表现出与ES细胞衍生的心肌细胞相似的分子和结构特征.
功能性评估揭示了iPS细胞心肌细胞中自发的Ca2+) 过渡物和功能性合,具有完整的β-上腺和肌肉信号传递.

结论:

iPS细胞成功地分化为功能性心肌细胞.
来自iPS细胞的自身功能性心肌细胞对细胞心肌形成术和心肌组织工程具有前景.
从iPS细胞衍生的心肌细胞为再生性心脏疗法提供了ES细胞的替代品.

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