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心血管细胞从小鼠诱导的多能干细胞中有针对性和系统的分化

Genta Narazaki¹, Hideki Uosaki, Mizue Teranishi

¹Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

|July 16, 2008

概括

诱导多能干细胞 (iPS) 可以系统地分化为各种心血管细胞,反映胚胎干细胞 (ES) 的行为. 这一进步有助于对iPS细胞生物学和心血管再生医学的理解.

科学领域:

干细胞生物学干细胞生物学
心血管研究的心血管研究.
再生医学是一种再生医学.

背景情况:

诱导多能干细胞 (iPS) 是通过重编程从体细胞生成的.
对于iPS细胞,详细的分化特性和有针对性的分化系统在很大程度上仍未被描述.

研究的目的:

为了研究小鼠iPS细胞向心血管血统的定向分化.
为了比较iPS细胞与胚胎干细胞 (ES) 的分化潜力.

主要方法:

为心血管细胞建立了小鼠ES细胞分化系统.
将该系统应用于iPS细胞,诱导表达Flk1的中皮细胞.
纯化的Flk1(+) 细胞和诱导的内皮细胞,壁细胞和心肌细胞系.

主要成果:

从iPS细胞中实现了各种心血管细胞的系统诱导.
差异化效率和时间表与小鼠ES细胞相似.
动脉,静脉,淋巴内皮细胞和心肌细胞成功生成.

结论:

iPS细胞表现出强大的分化到不同的心血管细胞类型.
iPS细胞的分化特性与ES细胞的分化特性非常相似.
该系统为iPS细胞生物学研究和心血管再生医学开发提供了巨大的潜力.

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相关概念视频

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.