核心N-甘段中的溶剂相互作用和构造选择:中心,分支的三甘单元及其单一水合复合物的气相构造
E Cristina Stanca-Kaposta1, David P Gamblin, Emilio J Cocinero
1Department of Chemistry, University of Oxford, Physical and Theoretical Chemistry Laboratory, South Parks Road, OX1 3QZ Oxford, United Kingdom.
Journal of the American Chemical Society
|July 18, 2008
概括
这项研究揭示了一个分支的三曼酸碳水化合物如何在隔离和水合时采用特定结构. 这种孤立的结构,具有强大的键,与其在水中的开放形式不同,突出显示了水.
科学领域:
- 碳水化合物化学和结构生物学.
- 葡萄糖生物学和分子相互作用.
背景情况:
- 甘酸对生物过程至关重要,其中核心糖体起着关键作用.
- 了解碳水化合物结构的隔离和溶液对于阐明它们的功能至关重要.
研究的目的:
- 为了研究一个有分支的trimannoside及其水合复合物的内在形状偏好.
- 将气相结构与水溶液中的结构进行比较,了解水的作用.
主要方法:
- 在低温下使用分子束膨胀进行气相隔离.
- 红外离子探光谱仪用于构造分析.
- ONIOM和MP2计算计算用于结构和能量确定.
主要成果:
- 无水化三曼诺酸采用一个紧的结构,由一个合作的键网络稳定.
- 单一的水合复合体保留了一个紧的结构,与水桥梁碳水化合物分支.
- 这与在水溶液中观察到的更开放,更受热的结构形成鲜明对比.
结论:
- 这项研究阐明了trimannosides在隔离与溶液中的独特形状偏好.
- 一个形状变化,涉及围绕α1,3) 糖链接的扭曲,允许水适应.
- 一个"水口袋"的形成表明,在N-糖甘水化中,中央曼诺斯单元的"切断"基团具有一般作用.
相关概念视频
Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Membrane Carbohydrates
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane Carbohydrates
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...


