来自古生物的F430变体的结构与甲无氧氧化相关
Stefan Mayr1, Christopher Latkoczy, Martin Krüger
1Laboratory of Organic Chemistry, ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland.
Journal of the American Chemical Society
|July 23, 2008
概括
研究人员在黑海微生物地毯中发现了一种新的含硫辅因子 (17(2) S) -17(2) -甲基-F430. 这种辅助因子对于甲 (AOM) 的无氧氧化至关重要,但在样本分离过程中可以形成人工物.
科学领域:
- 生物地质化学生物地质化学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 黑海中的微生物地毯使用硫酸盐对甲 (AOM) 进行无氧氧化.
- 这些地毯含有类似于甲基辅酶M减少酶的蛋白质,这表明甲在甲代谢中的作用.
研究的目的:
- 识别和描述黑海微生物地毯中参与AOM的含辅因子.
- 确定新型辅助因子的结构和配置,并调查它们的起源.
主要方法:
- 质谱学 (ICP-MS) 用于确定辅因子质量和元素组成.
- 高性能液态色谱 (HPLC) 用于净化辅因子和衍生物.
- 核磁共振 (NMR) 光谱用于结构阐明.
- 酸催化用于研究辅因子稳定性和人工物形成.
主要成果:
- 确定了两个含的辅因子:辅酶F430 (m/z = 905) 和一种新物种 (m/z = 951).
- 新型辅助因子被确定为 (17(2) S) -17(2) -甲基-F430五甲基,含有一个额外的硫原子.
- (17(2) S) 异构体是自然存在的形式,而更稳定的 (17(2) R) 异构体是在酸性隔离条件下形成的工件.
结论:
- 一种含硫的新型辅因子 (17(2) S) -17(2) -甲基-F430参与了海洋环境中的厌氧甲氧化.
- 需要仔细控制隔离条件,以避免人工物形成,并准确研究自然辅因子结构.
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