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在失败的人类心脏中增加心脏肌细胞前体

Hajime Kubo¹, Naser Jaleel, Asangi Kumarapeli

¹Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA.

|July 23, 2008

概括

研究人员在失败的人类心脏中发现心脏原生细胞增加,但这些细胞无法克服恶劣的心力衰竭环境. 这表明改善本地修复机制是心脏再生治疗的关键.

科学领域:

心血管生物学心血管生物学
再生医学是一种再生医学.
干细胞研究干细胞研究

背景情况:

成年哺乳动物的心脏具有内生修复机制.
这些机制对于维持心脏功能和应对损伤至关重要.

研究的目的:

研究人类心脏中心脏前代细胞的存在和功能.
为了比较失败与非失败的心脏中的祖细胞丰富度.
评估这些细胞对心脏肌细胞生成的潜力.

主要方法:

通过细胞分类从人类心脏中分离小c-kit+细胞.
多色流细胞计和免疫细胞化学用于细胞特征.
人类心脏原生细胞与新生小鼠心室肌细胞的共同培养.

主要成果:

从大多数人的心脏中分离出了小型c-kit+心脏原生细胞.
心脏衰竭显示,与心脏不衰竭相比,心脏原生细胞增加了近4倍.
孤立的细胞分化为新的心脏肌细胞,表现出电气合.

结论:

心脏原生细胞在失败的人类心脏中更为丰富,并且具有肌性能力.
失败的心脏环境似乎阻碍了内源性修复,需要移植.
未来的战略应集中于增强内在心脏再生过程.

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