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相关实验视频

Updated: Jul 3, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

两维组合选识别了特定的氨基糖体-RNA内部循环合作伙伴.

Matthew D Disney1, Lucas P Labuda, Dustin J Paul

  • 1Department of Chemistry, University at Buffalo, The State University of New York, and the New York State Center of Excellence in Bioinformatics and Life Sciences, 657 Natural Sciences Complex, Buffalo, New York 14260, USA. mddisney@buffalo.edu

Journal of the American Chemical Society
|July 26, 2008
PubMed
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相关概念视频

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

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这项研究通过使用一种新的二维组合查平台,确定了特定的RNA内部循环,这些循环与阿米诺甘油酸抗生素 (如neomycin B和tobramycin) 结合. 这些发现揭示了不同氨基糖化物更喜欢的独特的RNA循环结构,增强了对RNA-连接体相互作用的理解.

科学领域:

  • 分子生物学分子生物学
  • 化学生物学 化学生物学
  • 生物化学 生物化学

背景情况:

  • 氨基甘油酸抗生素对于治疗细菌感染至关重要.
  • 了解RNA-连接体相互作用是开发新疗法的关键.
  • 特定的RNA内部循环可以与小分子结合,影响生物活动.

研究的目的:

  • 确定特定的RNA内部环序列,这些序列与neomycin B,neamine,tobramycin和kanamycin A的衍生物结合.
  • 描述这些氨基糖化物和RNA内部环之间的结合特异性.
  • 为了证明二维组合选 (2DCS) 平台在RNA连接体发现方面的实用性.

主要方法:

  • 使用一个二维组合选 (2DCS) 平台.
  • 采用了一个在糖微阵列上固定的氨基糖化物库.
  • 测试了对3x3核酸RNA内部循环 (81,920个相互作用) 的图书馆的结合.
  • 通过凝切割收集结合的RNA进行分析.

主要成果:

  • 确定共识RNA内部循环用于neomycin衍生物 (GA对),tobramycin衍生物 (GG对) 和kanamycin A衍生物 (pyrimidine丰富的循环).

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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

相关实验视频

Last Updated: Jul 3, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

  • 尼氨基衍生品显示广泛的结合,包括Neomycin B衍生品也识别的循环.
  • 选择的内部循环对它们的相关氨基甘油酸具有很高的特异性,其特异性从2倍到80倍 (平均20倍) 不等.

    • 结论:

    • 2DCS平台通过同时探测RNA和化学空间,有效地识别了特定的RNA基因-连接体相互作用.
    • 独特的RNA内部循环结构与特定的氨基糖化物结合有关.
    • 这项工作为设计RNA向疗法和理解氨基糖化物-RNA相互作用提供了基础.