Jove
Visualize
联系我们

相关概念视频

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Engineered iMSCs delivering wild-type IL-2 achieve dose-sparing tumor control via CD25-dependent CD8<sup>+</sup> T-cell activation.

BMC medicine·2026
Same author

A comprehensive landscape of human organ N-glycoproteome.

Nature communications·2026
Same author

Amino-blocking sialic acid linkage specific alkylamidation for qualitative and quantitative analysis of sialylated glycoproteins.

Analytica chimica acta·2026
Same author

Integrative Quantitative Proteomics and N-Glycoproteomics Reveal Age-Processing of N-Glycosylation in Caenorhabditis elegans.

Proteomics·2026
Same author

Steering semi-flexible molecular diffusion model for structure-based drug design with reinforcement learning.

Science advances·2026
Same author

Ultrafast peptide preparation brings shotgun proteomics into the minute era.

Analytica chimica acta·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 3, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

电喷气电离是否会产生气相或液相结构?

Zhixin Tian1, Steven R Kass

  • 1Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Journal of the American Chemical Society
|July 30, 2008
PubMed
概括

电喷气对氨酸的电离化产生了基于溶剂的不同离子结构. 甲醇/水混合物有利于气相平衡,而乙二溶液主要产生碳酸盐离子.

科学领域:

  • 分析化学 分析化学
  • 物理化学 物理化学
  • 质谱测量质量谱测量

背景情况:

  • 电子喷射电离 (ESI) 是分析生物分子的关键技术.
  • 由ESI产生的离子的结构可以受到溶剂成分的影响.
  • 了解离子形成机制对于精确的质谱分析至关重要.

研究的目的:

  • 为了研究溶剂系统对气相异构组合产生的电喷离子化产生的铁酸脱质离子的溶剂系统的影响.
  • 为了确定观察到的离子比是否反映液相或气相平衡.

主要方法:

  • 使用了电子喷雾电离质谱法 (ESI-MS).
  • 氨酸被溶解在各种溶剂混合物中,包括甲醇/水和氨/水.
  • 分析了铁素的脱质离子 ([M-H]-),以确定它们的异构结构.

主要成果:

  • 在3:1的甲醇/水混合物中,氨酸的ESI产生了由70%的氧化物和30%的碳酸盐离子组成的脱质离子 ([M-H]-),反映了气相平衡.
  • 相比之下,无水性乙二和乙二/水混合物产生的主要是碳酸盐离子 (约. 95%) 的情况.
  • 将少量甲醇添加到基于乙尼特利的溶剂中,使离子组成重新转向气相平衡比率.

更多相关视频

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)
08:54

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)

Published on: June 8, 2011

相关实验视频

Last Updated: Jul 3, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)
08:54

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)

Published on: June 8, 2011

结论:

  • 电子喷射的氨酸离子的异构结构高度依赖于所使用的溶剂系统.
  • 在ESI-MS中选择溶剂可以显著影响观察到的离子组成,可能反映气相平衡而不是液相分布.
  • 这一发现对解释质谱数据和优化特定分析物的ESI条件有影响.