机械,寒冷和炎症性疼痛的细胞和分子基础
Bjarke Abrahamsen1, Jing Zhao, Curtis O Asante
1Molecular Nociception Group, University College London (UCL), Gower Street, London, WC1E 6BT, UK.
Science (New York, N.Y.)
|August 2, 2008
概括
表达通道Nav1.8的感觉神经元对于感知机械,寒冷和炎症疼痛至关重要. 然而,这些神经元对于感知热量或神经病痛的发展并不是必不可少的.
科学领域:
- 神经科学是一个神经科学.
- 疼痛研究 疼痛研究
- 分子生物学分子生物学
背景情况:
- 外围疼痛通路涉及各种感觉神经元,它们对各种刺激做出反应.
- 通道Nav1.8主要表达在涉及疼痛信号的特定感官神经元中.
研究的目的:
- 研究Nav1.8表达感官神经元在不同类型的疼痛感知中的特定作用.
- 为了确定Nav1.8表达神经元是否对急性,炎症和神经病痛至关重要.
主要方法:
- 利用白喉毒素选择性地消除表达Nav1.8.8的小鼠转移后感官神经元.
- 评估了对机械,热和冷刺激的反应中的疼痛行为.
- 在炎症性侮辱和神经损伤后评估疼痛反应.
- 在体内通过电生理学测量感官神经元的中央输入.
主要成果:
- 缺乏Nav1.8表达神经元的小鼠表现出正常的运动功能和对急性热的反应,但缺乏对有害机械压力和寒冷的反应.
- 这些小鼠在炎症性侮辱后表现出废除增强疼痛和自发疼痛行为.
- 神经损伤导致这些小鼠的疼痛敏感度增加,类似于对照 littermates.
- 电生理学记录证实了疼痛行为与中央感官神经元输入之间的相关性.
结论:
- Nav1.8-表达感官神经元对于机械,寒冷和炎症性疼痛感觉至关重要.
- 这些神经元不需要感知急性有害热或神经病痛的发展.
- 这些发现突出了Nav1.8通道对不同疼痛模式的特殊贡献.
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