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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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基于分子内移位的aptamer开关探测器

Zhiwen Tang1, Prabodhika Mallikaratchy, Ronghua Yang

  • 1Center for Research at the Bio/Nano Interface, Department of Chemistry, Shands Cancer Center and UF Genetics Institute, University of Florida, Gainesville, Florida 32611-7200, USA.

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|August 6, 2008
PubMed
概括

一个新的阿普坦转换探头 (ASP) 使用分子内信号传导进行敏感的分子检测. 这种基于aptamer的分子探针设计为目标识别提供了增强的光,显示了生物成像应用的巨大潜力.

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 分析化学 分析化学

背景情况:

  • 基于aptamer的探针对于分子检测至关重要.
  • 内部分子信号传导提供了一个敏感的检测机制.

研究的目的:

  • 为了展示一种新的基于aptamer的分子探针设计,利用分子内信号传导.
  • 设计和验证用于敏感和选择性目标检测的aptamer开关探测器 (ASP).

主要方法:

  • 设计了一种带有阿普坦,cDNA,PEG链接器,光体和火器的阿普坦转换探针 (ASP).
  • 为ATP和人类α-血目标设计的ASP.
  • 通过分子内信号传导对目标结合进行光变化的研究.

主要成果:

  • ASP的设计显示,在目标分子结合时光增强.
  • 由不同的度调解的量化光变化.
  • 对于ATP和人类α-血的标都获得了出色的选择性和快速反应.

结论:

  • 吸收器开关探头 (ASP) 设计可实现有效的分子内信号传导,用于分子检测.
  • 由于其灵敏度,稳定性和可重复使用性,ASP在生物芯片和现场成像中的应用具有有前途的潜力.