动态组合图书馆的脱血化是由 (-) -cytidine和 (-) -2-thiocytidine引起的
Mee-Kyung Chung1, Christine M Hebling, James W Jorgenson
1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3290, USA.
Journal of the American Chemical Society
|August 13, 2008
概括
一个动态库的基于酸的 dipeptides 变得脱血当绑定到像cytidine 这样的奇拉分析物. 这发生在特定受体的选择性放大过程中,在化学图书馆中表现出奇拉识别.
科学领域:
- 化学生物学 化学生物学
- 超分子化学 超分子化学
- 分析化学 分析化学
背景情况:
- 动态组合图书馆 (DCL) 为发现分子识别剂提供了一种强大的方法.
- 基质识别是化学的一个基本挑战,对制药和材料科学至关重要.
- 基于酸的化合物是超分子组件的多功能构建模块.
研究的目的:
- 为了研究一种基于racemichydrazone的 dipeptide库在与奇拉分析物结合时的脱血化.
- 阐明DCL内性识别和放大机制.
- 为了证明特定受体异构体的选择性结合和放大.
主要方法:
- 激光极度测量用于测量光学旋转.
- 质谱技术 (ESI-MS,LC-MS,MS/MS) 用于分子识别和量化.
- 高性能液态色谱 (HPLC) 和超高性能液态色谱 (UPLC) 用于分离和分析.
- 数字模拟以建模脱血化过程.
主要成果:
- 在与 (-) -cytidine和 (-) -2-thiocytidine的相互作用后,基于赛米的双库经历了脱血.
- 观察到 (SS) - 二聚体和 (RRRR) - 四聚体受体的选择性放大.
- 这些特定的受体优先结合性分析物,而不是它们的反体.
- 实验数据支持了选择性结合和放大拟议的机制.
结论:
- 基质分析剂可以通过受体放大在动态组合图书馆中诱导脱血.
- (SS) - 二聚体和 (RRRR) - 四聚体受体表现出高性选择性.
- 这项研究展示了一种使用DCL开发状传感器和选择器的方法.
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