基于SERRS的酶探针用于检测蛋白酶活性
Andrew Ingram1, Louise Byers, Karen Faulds
1Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.
Journal of the American Chemical Society
|August 15, 2008
概括
这项研究引入了一种新的方法,用于检测使用表面增强拉曼散射 (SERRS) 的蛋白酶活性. 这种技术允许实时分析,并为生物化学研究提供了一个新的工具.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 频谱学是一种光谱学.
背景情况:
- 蛋白酶活动在生物过程中至关重要.
- 目前用于蛋白酶检测的方法可能是有限的.
- 开发敏感和实时检测方法是必不可少的.
研究的目的:
- 开发和验证一种使用SERRS测量蛋白酶活性的新方法.
- 为了证明SERRS信号的"打开"和"关闭",以响应蛋白酶活动.
- 评估基质与各种蛋白酶的反应性,以便在现场分析.
主要方法:
- 一种新型的佐特醇染料基质与氨酸合成.
- 表面增强拉曼散射 (SERRS) 被用作检测技术.
- 通过SERRS信号变化监测基质的蛋白酶诱导裂变.
主要成果:
- 通过合成氨酸的结合,SERRS信号被成功关闭了.
- 蛋白酶活动恢复了SERRS信号,使检测成为可能.
- 在不同的蛋白酶中观察到不同的基质活性.
- 对于Subtilisin carlsberg,一个检测极限为50 ng ml-1.
结论:
- SERRS是一种可行的新方法,用于量化蛋白酶活性.
- 开发的基板允许对蛋白酶进行敏感的实时和现场监测.
- 这种方法为生物化学和诊断应用提供了一个有前途的工具.
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