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相关概念视频

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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相关实验视频

Updated: Jul 2, 2026

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

由腺病毒e1a进行表观遗传重编程.

Roberto Ferrari1, Matteo Pellegrini, Gregory A Horwitz

  • 1Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.

Science (New York, N.Y.)
|August 23, 2008
PubMed
概括
此摘要是机器生成的。

亚地诺病毒e1a蛋白通过改变基因组乙化和抑制抗病毒反应来重新编程人体细胞. 这种表观遗传重编程刺激细胞循环,促进细胞转化.

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Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
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Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Published on: October 26, 2018

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
11:00

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Published on: September 9, 2010

相关实验视频

Last Updated: Jul 2, 2026

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
08:14

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Published on: October 26, 2018

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
11:00

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Published on: September 9, 2010

科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学
  • 病毒学 病毒学

背景情况:

  • 众所周知,腺病毒e1a蛋白在静止的人类细胞中诱导细胞增殖.
  • 通过e1a实现这种细胞重编程的精确分子机制尚未完全理解.

研究的目的:

  • 调查腺病毒e1a在人类细胞表观遗传重编程中的作用.
  • 阐明e1a与关键调节蛋白相互作用的时间动态及其对基因表达的影响.

主要方法:

  • 染色体免疫沉 (ChIP) 用于分析基因促进体的蛋白质结合和基因素修饰.
  • 西部涂抹测试以评估蛋白质水平和基因素乙化.
  • 基因表达分析以确定转录变化.

主要成果:

  • 腺病毒e1a导致视网膜母细胞瘤 (RB) 蛋白和p300/CBP基因组乙烯转移酶的全球重新定位.
  • e1a限制3素-18乙化 (H3K18ac) 基因组在有限的基因组中,刺激细胞循环并抑制抗病毒反应.
  • 对e1a与细胞循环,抗病毒和分化基因的促进体的时间结合协调了转录性变化.

结论:

  • 腺病毒e1a通过定义的表观遗传重编程过程驱动细胞转化.
  • e1a与p300/CBP和RB蛋白的时间相互作用对其功能至关重要.
  • 了解这些机制,可以了解病毒瘤发生和细胞转化.