异染色素完整性会影响在中粒体功能失调后的染色体重组
Kojiro Ishii1, Yuki Ogiyama, Yuji Chikashige
1Division of Cell Biology, Institute of Life Science, Kurume University, Japan.
Science (New York, N.Y.)
|August 23, 2008
概括
酵母菌中中心基因功能障碍导致幸存者具有新中心基因或融合的无中心染色体. RNA干扰会影响这种染色体重组,影响型进化.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 中心质对真核染色体遗传和基因组稳定性至关重要.
- 中心体的表观遗传调节与基因组稳定性,型进化和物种化有关.
- 中心细胞功能障碍对染色体结构的影响仍然不太清楚.
研究的目的:
- 为了研究中粒体功能障碍在Schizosaccharomyces pombe中的染色体后果.
- 探索表观遗传机制的作用,特别是RNA干扰,在与中心粒相关的染色体重排中的作用.
主要方法:
- 在Schizosaccharomyces pombe.中删除中间体的条件.
- 对幸存者染色体进行分析,以确定新中心粒形成和端粒融合.
- 参与RNA干扰依赖的异染色体形成的基因的非激活.
主要成果:
- 脱离中间体的幸存者表现出在分端体区域的新中心体获取或通过端体融合的中枢染色体救援.
- RNA干扰途径基因的失活显著降低了新中心体形成与端粒融合的比率.
- 异染色素的基因组分布影响了染色体重组的模式.
结论:
- 中心粒体功能障碍会触发不同的染色体重组途径:新中心粒体形成或端粒体间融合.
- 通过RNA干扰介导的异染色素在调节这些重组路径方面发挥着关键作用.
- 异色染色素的分布模式可能是指导型进化的关键因素.
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