在有机发生过程中,相反的Wnt通路引导细胞极性
Jennifer L Green1, Takao Inoue, Paul W Sternberg
1Howard Hughes Medical Institute/Division of Biology, California Institute of Technology, Mail Code 156-29, Pasadena, CA 91125, USA.
Cell
|August 30, 2008
概括
组织极性依赖于整合多个Wnt通路. 在C. elegans vulva中,不对称的细胞分裂使用Wnts来定位细胞并通过新的信号机制建立基极性.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 不对称的细胞分裂对组织和器官组织至关重要.
- 在C. elegans vulva发育中,镜像对称性是通过对立的前体细胞 (VPC) 的分裂方向来实现的.
研究的目的:
- 描述控制C. elegans中VPC的不对称分裂模式的分子机制.
- 阐明多个Wnt信号通路如何有助于建立组织极性.
主要方法:
- 对Wnt信号组件 (MOM-2,LIN-44,EGL-20) 和它们的受体 (Fz/LIN-17,Ryk/LIN-18,CAM-1,VANG-1) 的分析.
- 研究β-catenin局部化和基因转录激活.
- 研究细胞"地面"极性的建立.
主要成果:
- 在对称轴上表达的 Wnts MOM-2 和 LIN-44,通过 Fz/LIN-17 和 Ryk/LIN-18 路径将 VPC 中心定向.
- 通过Wnt/EGL-20信号来建立一个统一的"地面"极性.
- EGL-20利用了一种涉及Ror受体氨酸激酶CAM-1和平面细胞极性成分Van Gogh/VANG-1的新途径.
结论:
- 组织极性是由不同的Wnt信号通路的整合决定的.
- 多个Wnt通路合作控制细胞分裂方向,并在阴道发育过程中建立极性.
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