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检测单分子DNA杂交,使用酶放大在一系列的femtoliter大小的反应容器中的单分子DNA杂交

Zhaohui Li1, Ryan B Hayman, David R Walt

  • 1Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA.

Journal of the American Chemical Society
|September 4, 2008
PubMed
概括

这项研究引入了一种灵敏的单分子DNA测定平台,达到1个女性分子检测极限. 新型的五升井阵列和酶放大使生物医学应用中高精度的DNA检测成为可能.

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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...