合成DNA-有机分子-DNAtriblock寡合物使用胺基合反应及其酶增强酶的合成
Jungkyu K Lee1, Young Hwan Jung, Randall M Stoltenberg
1Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Journal of the American Chemical Society
|September 4, 2008
概括
研究人员开发了一种简单的方法,使用DNA创建DNA-有机分子-DNA结构,用于单分子电子. 这种DNA模板方法为纳米设备和生物传感器提供了精确的电接触.
科学领域:
- 分子电子学分子电子学
- 纳米技术纳米技术
- 生物结合化学的化学
背景情况:
- 精确的电接触对于单分子电子,包括生物传感器和纳米设备至关重要.
- DNA的纳米尺寸和长度使其成为纳米电子应用的合适模板.
- 开发可靠的方法来将分子连接到电极对于推动分子电子学的发展至关重要.
研究的目的:
- 研究胺合的反应性,用于将寡度氧核化物 (ODN) 与有机分子结合.
- 通过聚合酶链反应 (PCR) 合成DNA-有机分子-DNA (DOD) 三块架构.
- 建立一个平台,准备和研究单分子电子.
主要方法:
- 胺基合反应将ODN与有机分子结合起来.
- 聚合酶链反应 (PCR) 用于DNA延长.
- 电子喷雾电离质谱仪 (ESI-MS) 用于确认合反应.
- 糖凝电泳和原子力显微镜 (AFM) 用于结构特征.
主要成果:
- 通过氨基酸合成功将ODN与有机分子结合起来.
- 1.5 kbp dsDNA有机分子的合成-1.5 kbp dsDNA三块架构的合成.
- 确认成功合成并对国防部结构进行表征.
结论:
- 开发的策略为构建DNA-有机分子-DNA三块结构提供了一种简单的方法.
- 这种方法为单分子电子的准备和研究提供了一个潜在的平台.
- 该研究表明,使用DNA作为模板来创建用于电子应用的功能纳米结构的可行性.
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