DNA聚合酶催化了DNA网络的增长
Sascha Keller1, Jie Wang, Madhaviah Chandra
1Department of Chemistry and Konstanz Research School Chemical Biology, Universität Konstanz, 78457 Konstanz, Germany.
Journal of the American Chemical Society
|September 11, 2008
概括
研究人员使用DNA聚合酶链反应 (PCR) 创建了3DDNA网络. 这种方法允许可定制的网络结构和功能组的添加,为纳米技术应用提供了一个多功能平台.
科学领域:
- 生物技术是生物技术.
- 纳米技术纳米技术
- 分子生物学分子生物学
背景情况:
- DNA独特的基配对特性使得精确的分子相互作用成为可能.
- DNA纳米技术利用这些特性来构建纳米级架构.
- 有针对性的自我组织对于创建复杂的纳米结构至关重要.
研究的目的:
- 开发一种用于构建基于3D DNA的网络的方法.
- 通过PCR使这些DNA网络的放大成为可能.
- 为调整网络属性和引入功能提供灵活的平台.
主要方法:
- 利用DNA作为自我组织的支架.
- 采用DNA聚合酶链反应 (PCR) 进行网络生成和放大.
- 改变模板大小以控制网络网状网状尺寸.
- 在PCR过程中包含化学修饰的核酸.
主要成果:
- 成功构建基于3D DNA的网络.
- 用PCR放大来生成这些网络的演示.
- 可调节网络网格尺寸通过改变模板尺寸来实现.
- 通过改性核酸引入功能和记者部分.
结论:
- 开发的基于PCR的方法为创建可调节的3DDNA网络提供了一种多功能方法.
- 这种技术允许结合多种功能,扩大纳米技术中的应用.
- 放大DNA网络的能力为纳米结构制造提供了一个可扩展的路线.
相关概念视频
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
01:31Proofreading
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore, it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Errors During Replication are Corrected by the DNA Polymerase Enzyme
01:43Proofreading
Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
02:10Translesion DNA Polymerases
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
02:40DNA Replication
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Replication in Prokaryotes
DNA replication uses a large number of...