在C. elegans中进行转接,产生负RNAi调节器ERI-6/7
Sylvia E J Fischer1, Maurice D Butler, Qi Pan
1Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Nature
|September 12, 2008
概括
研究人员在Caenorhabditis elegans中发现了eri-6和eri-7基因的突变,揭示了一个新的RNAi通路调节器. 这种螺旋酶蛋白控制着外源和内源RNA干扰通路.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- RNA干扰 (RNAi) 途径利用小RNA来调节基因表达.
- 影响RNAi组件的突变可以揭示通路相互作用和调节机制.
研究的目的:
- 为了确定Caenorhabditis elegans中小RNA通路的新型调节者.
- 为了研究参与RNA干扰的新发现基因的功能.
主要方法:
- 对Caenorhabditis elegans突变的查,这些突变对双链RNA (dsRNA) 的反应增强.
- 基因补充分析以确定eri-6和eri-7中的突变.
- 对前信使RNA (pre-mRNA) 处理的分析,包括转接和腺转化为 inosine 的编辑.
- 描述ERI-6/7蛋白作为超级家族I酶的特征.
主要成果:
- 在两个相邻的,分歧转录的基因eri-6和eri-7中分离突变.
- 发现eri-6和eri-7通过转接形成单一的功能mRNA (eri-6/7).
- 证据表明,在拼接之前,通过腺到氨酸编辑形成的双链前mRNA中间体.
- ERI-6/7蛋白质是一种酶,对外源RNAi进行负调节,并参与内源RNAi.
结论:
- 埃里-6/7基因位点编码了RNA干扰通路的关键调节器.
- 核前mRNA编辑和转接是eri-6/7 mRNA成熟的关键步骤.
- ERI-6/7螺旋酶在控制外来RNAi和自衍生的RNAi方面发挥着双重作用.
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