相关实验视频

Updated: Feb 3, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

卵孔和偏头痛的专利:来自北曼哈顿研究 (NOMAS) 的横截面研究

Tatjana Rundek¹, Mitchell S V Elkind, Marco R Di Tullio

¹Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA. trundek@med.miami.edu

|September 17, 2008

概括

在一项基于人口的大型研究中,形孔 (PFO) 与偏头痛无关. 这一发现质疑PFO关闭对于未经选择的偏头痛患者的作用.

科学领域:

心脏病学心脏病学
神经学神经学
流行病学流行病学

背景情况:

有一种假设的联系存在于 patent foramen ovale (PFO) 和偏头痛之间.
据报道,穿皮式PFO闭合改善了偏头痛的频率和严重程度.
关于PFO-偏头痛关系的基于人口的数据有限.

研究的目的:

在没有中风的个体中调查PFO和偏头痛之间的联系.
分析来自多民族,城市,基于人口的队列的数据.
评估PFO流行率及其与自我报告的偏头痛的相关性.

主要方法:

从北曼哈顿研究 (NOMAS) 中评估了1101名没有中风的受试者.
偏头痛病史是自我报告的;PFO的存在是通过胸前回声心电图确定的.
使用多变量逻辑回归来分析相关性,并对共变量进行调整.

主要成果:

偏头痛的患病率为16%,PFO的患病率为15%.
在患有和没有偏头痛的受试者之间没有发现PFO患病率的显著差异 (14.6%与15.0%,P=0.9).
调整后的分析显示,PFO和偏头痛之间没有关联 (OR 1.01,95% CI 0.63-1.61).

结论:

通过心声学检测到的PFO在这个老年多民族队列中与自我报告的偏头痛无关.
PFO和偏头痛之间的因果关系仍然不确定.
在未经选择的偏头痛患者中,PFO关闭的实用性是可疑的.

更多相关视频

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Published on: December 23, 2016

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

相关概念视频

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8⁺ T Cells During Tumorigenesis

The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T...

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated...

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8⁺ T Cell Responses

We describe here a flow cytometry-based in vivo killing assay that enables examination of immunodominance in cytotoxic T lymphocyte (CTL) responses to a model tumor antigen. We provide examples of how this elegant assay may be employed for mechanistic studies and for drug efficacy...

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Analysis of Simian Immunodeficiency Virus-specific CD8⁺ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Here, we present an optimized protocol for enumerating and characterizing rhesus macaque CD8+ T cells against the AIDS virus. This article is useful not only to the field of HIV immunology, but also to other areas of biomedical research where CD8+ T cell responses are known to affect disease...

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

We present a flow cytometry-based method to examine T cell development in vivo using genetically manipulated mice on a wildtype or T cell receptor transgenic...