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Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

化学. 在化学. 标签不粘 混乱的化学生物学问题

Dennis P Curran1

  • 1Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15208, USA. curran@pitt.edu

Science (New York, N.Y.)
|September 20, 2008
PubMed
概括

No abstract available in PubMed .

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...