分子内应变协调基因在微管道沿着微管道的步行行为
Ahmet Yildiz1, Michio Tomishige, Arne Gennerich
1Department of Cellular and Molecular Pharmacology and the Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.
Cell
|September 23, 2008
概括
素运动蛋白利用链体的张力来协调它们的两个运动领域,以便在微管道上有效地运动. 降低这种张力会损害步行,而外部力量甚至可以在没有ATP的情况下诱导运动.
科学领域:
- 分子运动功能的分子运动功能.
- 细胞力学 细胞力学
- 生物物理学的生物物理.
背景情况:
- 素电机沿着微管子移动,由ATP水解提供动力.
- 精确的协调素两个运动域的机制是未知的.
- 子链接器被假设通过分子内部张力来调解这种协调.
研究的目的:
- 为了研究由链器产生的分子内部张力在动力协调中的作用.
- 为了确定应变是否影响ATP水解和电机步进之间的合.
主要方法:
- 基因素运动域是通过使用人工来设计的延长链.
- 通过光学陷应用外部张力和没有外部张力来测量运动活动.
- 分析了修改后的基因素结构的步进行为.
主要成果:
- 通过延长链器来降低分子内部紧张,损害了ATP水解和向前迈进之间的协调,降低了运动速度.
- 使用光学陷施加外部张力恢复了接近正常的电机转速.
- 外部负荷诱导了双向踏步,在一个不动的运动中缺乏链和ATP.
结论:
- 激素的运动领域感知并响应机械应力.
- 链条产生的分子内张力对于高效的动态步进和域间通信至关重要.
- 机械力量在调节运动和运动功能的过程中起着重要作用.
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