在裂变酵母中,促进器驱动的拼接调节
Alberto Moldón1, Jordi Malapeira, Natalia Gabrielli
1Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/Doctor Aiguader 88, Barcelona 08003, Spain.
Nature
|September 26, 2008
概括
在裂变酵母中,Rem1是一种化特异性循环蛋白,通过转录和拼接来调节. Mei4和Fkh2控制其表达,影响介质重组和细胞周期进展.
科学领域:
- 分子生物学分子生物学
- 细胞循环规则 细胞循环规则
- 酵母遗传学 酵母遗传学
背景情况:
- 介质期与线粒期的区别在于介质期前的S阶段,两个分裂,以及独特的染色体分离.
- 雷姆1是 * Schizosaccharomyces pombe * 中的一种化特异性环林,对于重组和及时的化I开始至关重要.
- 宫外Rem1表达导致G1停止和植物细胞中的线粒性灾难.
研究的目的:
- 研究转录和转录后层次的 *rem1* 基因表达的调节机制.
- 阐明特定转录因子在控制*rem1*拼接和功能中的作用.
主要方法:
- 通过转录和拼接对*rem1*基因调节的分析.
- 构建和分析含有*rem1*促进子与其他含内子基因融合的仿真基因.
- 研究分叉转录因子Mei4和Fkh2.2的参与.
主要成果:
- *rem1*表达是由转录和替代拼接控制的,产生不同的蛋白质.
- *rem1*的拼接规则独立于其转录的区域.
- Mei4促进*rem1*的转录和拼接,而Fkh2则在植物生长和S阶段介导细胞内保留.
结论:
- *rem1* 拼接是特定于 meiotic 的,并由 Mei4 和 Fkh2.2 调节.
- Fkh2在调节*rem1*剪接中起着双重作用,在特定的细胞周期阶段促进内质保留.
- 这些发现揭示了一种新的基因调节层,对S. pombe*中介性进展至关重要.
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