功能模块的保存和重新连接,由裂变酵母酵母的表皮图揭示出来
Assen Roguev1, Sourav Bandyopadhyay, Martin Zofall
1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
Science (New York, N.Y.)
|September 27, 2008
概括
研究人员绘制了裂变酵母中的基因相互作用图,发现了一个新的RNA干扰 (RNAi) 组件,并揭示了酵母物种之间保存的功能模块. 这为染色体功能和遗传网络的进化保护提供了洞察力.
科学领域:
- * 分子和系统生物学
- * 遗传学和基因组学
- * 酵母作为一个模型生物体
背景情况:
- * 了解基因功能和生物通路依赖于绘制基因相互作用.
- *表观图绘制 (E-MAP) 提供了这些相互作用的高分辨率视图.
- * 跨物种的比较分析可以揭示保存和分离的生物机制.
研究的目的:
- * 构建一个定量表征图 (E-MAP) 在 * Schizosaccharomyces pombe * (裂变酵母).
- * 调查与染色体功能相关的遗传相互作用,包括转录,DNA修复和复制.
- *将裂变酵母E-MAP与芽酵母的现有数据进行比较,以了解遗传网络的进化保护.
主要方法:
- *对所有对对组合的突变进行表型的系统测量.
- *高密度定量基因相互作用映射.
- *对S. pombe和芽酵母之间的遗传相互作用概况进行比较分析.
主要成果:
- *为S. pombe*构建一个全面的E-MAP,重点是染色体生物学.
- *确定了RNA干扰 (RNAi) 途径的一个新组成部分,Rsh1.
- *证明功能模块 (蛋白质复合体) 显示保存的遗传相互作用,而模块之间的交叉交谈可能不同.
结论:
- *这项研究扩大了对RNAi机制及其与其他细胞过程的整合的理解.
- * 遗传相互作用的保存在蛋白质复合体水平上是明显的.
- * E-MAP 的比较分析突出了酵母菌种之间遗传结构的保存和分歧方面.
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