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通过电子断层扫描揭示的 FcRn 介导的抗体穿越上皮细胞的运输
Wanzhong He1, Mark S Ladinsky, Kathryn E Huey-Tubman
1Division of Biology 114-96, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA.
Nature
|September 27, 2008
概括
新生儿的Fc受体 (FcRn) 通过肠道转细胞酶促进母亲的IgG传输到新生儿. 这项研究可视化了IgG通过复杂的囊泡网络的运动,揭示了这一关键免疫转移过程的新细节.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 新生儿Fc受体 (FcRn) 对于将母体IgG传递给新生儿至关重要,为新生儿提供被动免疫.
- FcRn通过依赖pH的结合和细胞转移来调解肠道上皮细胞的IgG运输.
- 了解FcRn介导的IgG转细胞化的精确机制对于新生儿免疫是至关重要的.
研究的目的:
- 为了可视化和描述FcRn介导的IgG转细胞的空间和时间动态,在新生小鼠皮上皮细胞.
- 阐明涉及IgG运输的复杂囊泡网络和贩运途径.
主要方法:
- 使用纳米黄金标记Fc和免疫标记的电子断层扫描来可视化FcRn介导的IgG传输.
- 采用非扰乱性内细胞标签来识别受体结合的配体和特征运输囊泡.
- 结合成像技术,通过相互连接的囊泡追踪IgG的运动,并识别微管关联.
主要成果:
- 通过复杂的管状和不规则囊泡网络可视化IgG运输,其中一些与微管相关.
- 鉴定了转细胞体的新方面,包括多细胞体内囊泡/管体的参与和克拉特林涂层坑外细胞体.
- 通过早期,晚期和循环内分泌体标记,通过早期,晚期和循环内分泌体标记显示出明显的和重叠的囊泡标记,表明复杂的内分泌体-溶解体贩运.
结论:
- FcRn介导的IgG转细胞化涉及一个复杂的,动态的囊泡和内体区网络.
- 获得了有关IgG运输中的囊泡形态多样性和贩运途径的新见解.
- 这项研究提供了IgG转细胞化的详细时空地图,进步了我们对新生儿幽默免疫力的理解.
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