在心肌停止和再输液过程中,在人类心肌间歇体内发生矩阵金属蛋白酶活性的动态变化
Francis G Spinale1, Christine N Koval, Anne M Deschamps
1Cardiothoracic Surgery, Strom Thurmond Research Center, 114 Doughty St, Suite 625, Charleston, SC 29425, USA.
Circulation
|October 10, 2008
概括
在人类心脏间隙中,矩阵金属蛋白酶 (MMP) 的活性是动态的. 这项研究表明,在心脏骤停后的再注血后,MMP间歇性活性显著增加.
科学领域:
- 心血管生物学 心血管生物学
- 酶学 是一种酶学.
- 心肌病理生理学 心肌病理生理学
背景情况:
- 矩阵金属蛋白酶 (MMPs) 涉及到不良的心肌重塑后缺血/再输液.
- 之前的研究依赖于提取的样本,使得人心肌中的现场间歇性MMP活性未知.
研究的目的:
- 在缺血和再注射期间,直接量化人类心肌中的间歇性矩阵金属蛋白酶 (MMP) 活性.
- 为了研究心肌MMP活动的动态变化在心脏手术期间,涉及心肺旁路.
主要方法:
- 使用微透析系统与验证的MMP化基质放置在14名患者的心肌内.
- 在稳定状态,心脏骤停和再注射期间,使用线内光检测持续监测间歇性MMP活性.
- 在心肺绕道和分离过程中,与基线值相比,MMP活性的测量变化.
主要成果:
- 间歇性MMP活性在微透析系统部署后10分钟内达到稳定的基线.
- 在心肺绕道开始时,MMP活性增加了20%,然后随心脏骤停而下降.
- 在再输血和绕道分离后,间歇性MMP活性比基线增加了30%以上.
结论:
- 直接证明了在人类心肌间歇体内的矩阵金属蛋白酶 (MMP) 蛋白质分解活性的存在和动态性质.
- 表明心肌止和再注血在体内显著改变间歇性MMP活性.
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