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Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
Published on: January 4, 2011
猿类和人类疟疾寄生虫Plasmodium knowlesilesi的基因组
1Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK. ap2@sanger.ac.uk
Nature
|October 10, 2008
概括
据了解,Plasmodium knowlesi基因组揭示了分散的变异抗原基因,与其他Plasmodium物种不同. 这只子是疟疾寄生虫.
科学领域:
- 疟疾学 疟疾学
- 基因组学就是基因组学.
- 寄生虫学的寄生虫学
背景情况:
- 疟原虫 (Plasmodium knowlesi) 是一种子疟疾寄生虫,在东南亚越来越多地引起人类感染.
- 它与Plasmodium vivax有着家族遗传关系,但表现出不同的表型特征.
- 抗原变异首次被证明是在Plasmodium knowlesi.中.
研究的目的:
- 介绍Plasmodium knowlesi (H菌株,Pk1(A+) 克隆) 的核基因组序列分析.
- 为了能够与Plasmodium vivax和其他Plasmodium物种进行比较基因组学.
- 研究P. knowlesi.中的抗原变异的基因组基础.
主要方法:
- 整个基因组测序的Plasmodium knowlesi (H菌株,Pk1(A+) 克隆).
- 核基因组序列的生物信息分析.
- 与其他Plasmodium物种进行基因组比较分析.
主要成果:
- 这是第一次描述子疟疾寄生虫的基因组序列.
- 假定变异抗原家族分布在整个P. knowlesi基因组中,与端粒重复有关.
- 变异抗原的KIR家族显示了与宿主CD99细胞外域的序列同质性,这表明分子模拟.
结论:
- P. knowlesi 的基因组为与其他类型的原菌进行比较研究提供了宝贵的资源.
- 在P. knowlesi中,变异抗原家族的基因组组织与其他Plasmodium物种有显著差异.
- 由KIR序列所暗示的分子模拟可能代表了Plasmodium knowlesi.中的一种新型免疫逃避策略.
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