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通过开放的Na(+),K(+) -ATPase的离子通路

Ayako Takeuchi¹, Nicolás Reyes, Pablo Artigas

¹Laboratory of Cardiac/Membrane Physiology, The Rockefeller University, New York, New York 10065, USA.

|October 14, 2008

概括

毒素通过创建离子运输的连续通道,将-转化为离子通道. 这一发现揭示了在相关的P型ATPases中保留的阴离子通路.

科学领域:

膜蛋白结构和功能膜蛋白结构和功能
离子运输机制离子运输机制
细胞电生理学细胞电生理学

背景情况:

P型ATPases对于维持细胞电化学梯度至关重要.
通常,这些中的离子结合点在膜侧面之间交替接入,以防止梯度消散.
毒素破坏了Na(+),K(+) -ATPase中的这种交替,将转化为离子通道.

研究的目的:

为了研究与毒素结合的Na(+),K(+) -ATPase通道中的离子透的结构基础.
为了确定形成离子通路的特定氨基酸残留物.
为了探索相关的P型中这种路径的保护.

主要方法:

通过Na(+),K(+) -ATPase的跨膜螺旋体 (TM1-TM6) 进行囊扫描突变发生.
使用水溶性硫烯特异性试剂在通道内探测可访问性.
电生理记录用于监测离子流和试剂可访问性.

主要成果:

一个单一的,不间断的阴离子通路被确定跨越膜在毒素结合的管道.
这一途径涉及TM1,TM2,TM4和TM6的残留物,包括离子结合部位II.
该通道可以从膜的两侧访问,与通道活动一致.

结论:

毒素的结合通过Na(+),K(+) -ATPase创建了一个连续的离子透通道.
这种途径很可能保留在其他P型ATPases中,例如Ca2+) -ATPases和H+),K+) -ATPases.
了解这种途径可以了解P型ATPase结构和离子通道功能.

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