玉米的Viviparous-1发育基因编码了一个新的转录激活器
D R McCarty1, T Hattori, C B Carson
1Vegetable Crops Department, University of Florida, Gainesville 32611.
Cell
|September 6, 1991
概括
玉米的Viviparous-1 (Vp1) 基因编码了一个新型的转录因子,对种子成熟至关重要. 这种蛋白质激活基因表达,这表明它在种子发育期间在植物激素反应中起作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 玉米中的Viviparous-1 (Vp1) 基因对于调节种子成熟至关重要.
- 了解Vp1的分子机制是破译种子发育途径的关键.
研究的目的:
- 描述Vp1基因产物并阐明它在玉米种子发育中的功能.
- 确定负责转录激活的Vp1蛋白的功能域.
主要方法:
- Vp1基因克隆和蛋白质表征.
- 将Vp1与GAL4DNA结合域融合以确定激活序列.
- 在玉米原生质中进行记者基因测定,以评估交换活化潜力.
主要成果:
- Vp1编码了一个73,335达尔顿的蛋白质,缺乏与已知蛋白质的同质性.
- 在Vp1.1中确定了一个酸性转录激活域.
- 在玉米原生质中,VP1强烈激活了记者基因表达 (>130倍).
- 酸性域对于事务激活是必不可少的,并且可以通过VP16激活器序列进行功能替代.
结论:
- 在玉米中,VP1作为一种新型的转录因子.
- 已识别的酸性域对Vp1的交换活化活动至关重要.
- 在发育过程中,Vp1可能会增强种子特异性激素反应.
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